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- The plates were incubated with the viability reagent for 2 h and the absorbance of the color complex measured at 490 nm with a reference wavelength set at 690 nm for XTT using KC Junior software and a BIO-TEK Power-Wave XS multi-well plate reader (A.D.P, Weltevreden Park, South Africa). The IC50 values were calculated using GraphPad Prism 4.
